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كتاب Protein Arrays Methods and Protocols لEric T Fung MD PhD

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المؤلف : Eric T Fung MD PhD
التصنيف : كتب منوعة
الفئة : Biology Books
سنة النشر : 2004
عدد الصفحات : غير محدد
عن الكتاب : 2004م - 1443هـ نبذه عن الكتاب: Protein Arrays From cDNA Expression Libraries Hendrik Weiner, Thomas Faupel, and Konrad Büssow Summary This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the array for binding proteins. After producing the library and decollating the expression clones, one can pick thousands of expression clones with a laboratory robot and can deposit them into microtiter plates in an ordered manner. Such ordered clone libraries are the starting material for the construction of a high-density protein array. This array is constructed by spotting the expression clones onto a protein-binding membrane. Following cell growth and induction of protein expression on the membrane, the cell spots are lysed and their recombinant protein immobilized on the membrane. The so-constructed array carries thousands of proteins without the need to clone, express, and spot individual proteins. Such arrays allow one to screen for numerous protein functions in a high-throughput manner. Key Words: Protein array; cDNA expression library; high-density spotting; clone array; protein antigen; protein function; protein–protein interaction; posttranslational modification; high-throughput screening. 1. Introduction Arrays of complementary DNA (cDNA) expression libraries carry thousands of proteins without the need to clone, express, and spot individual proteins (1). These arrays are practical formats to screen en masse for a given protein function, that is, to identify protein antigens (1,2), including autoantigens (3), binding proteins (4), and substrates for arginine methyltransferases (5). Although not yet demonstrated, the arrays may also permit studies on posttranslational modifications other than protein methylation, that is, to find substrates for certain protein kinases. The protein arrays described here are made using cDNA libraries that are constructed in expression vectors. With the help of a laboratory robot, one can pick thousands of library clones and can deposit them into microtiter plates in an ordered .
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نبذة عن كتاب Protein Arrays Methods and Protocols

كتاب Protein Arrays Methods and Protocols

2004م - 1443هـ نبذه عن الكتاب: Protein Arrays From cDNA Expression Libraries Hendrik Weiner, Thomas Faupel, and Konrad Büssow Summary This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the array for binding proteins. After producing the library and decollating the expression clones, one can pick thousands of expression clones with a laboratory robot and can deposit them into microtiter plates in an ordered manner. Such ordered clone libraries are the starting material for the construction of a high-density protein array. This array is constructed by spotting the expression clones onto a protein-binding membrane. Following cell growth and induction of protein expression on the membrane, the cell spots are lysed and their recombinant protein immobilized on the membrane. The so-constructed array carries thousands of proteins without the need to clone, express, and spot individual proteins. Such arrays allow one to screen for numerous protein functions in a high-throughput manner. Key Words: Protein array; cDNA expression library; high-density spotting; clone array; protein antigen; protein function; protein–protein interaction; posttranslational modification; high-throughput screening. 1. Introduction Arrays of complementary DNA (cDNA) expression libraries carry thousands of proteins without the need to clone, express, and spot individual proteins (1). These arrays are practical formats to screen en masse for a given protein function, that is, to identify protein antigens (1,2), including autoantigens (3), binding proteins (4), and substrates for arginine methyltransferases (5). Although not yet demonstrated, the arrays may also permit studies on posttranslational modifications other than protein methylation, that is, to find substrates for certain protein kinases. The protein arrays described here are made using cDNA libraries that are constructed in expression vectors. With the help of a laboratory robot, one can pick thousands of library clones and can deposit them into microtiter plates in an ordered .


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