أعلان
تحميل كتاب Principles and Protocols Humana Press pdf
المؤلف : Marilena Aquino de Muro Ralph Rapley
عن الكتاب : 2001م - 1443هـ نبذه عن الكتاب:
The isolation of specific regions within the genome of an organism is now
normally accomplished by polymerase chain reaction (PCR) amplification
using primers specific for the region in question. However, there are occasions
where this is not possible (loss of primer sites resulting in no amplification or if
there are no primers available). In these cases the detection of the sequence of
interest can be achieved by hybridization of a labeled probe to restricted
genomic DNA immobilized on a membrane by Southern blotting (1). Genomic
DNA is first digested by one or more restriction enzymes and the fragments
generated separated by gel electrophoresis. The amount of DNA to be applied
to the gel varies from application to application. In general 10 µg of human
genomic DNA is needed for the detection of a single copy gene when using
radioactively labeled probes and an overnight exposure to X-ray film. This
figure can be reduced if the target is either a repetitive element (e.g., ribosomal
DNA) or, if plasmid DNA or PCR products are run on the gel. Once the fragments are separated on the gel the DNA is then denatured in situ and transferred by capillary transfer to either a nitrocellulose or nylon membrane. The
DNA fragments are then bound to the membrane, which can then be used in a
hybridization reaction.
1.2. Materials
1.2.1. Specific Materials
1. 3MM filter paper (Whatman), paper towels, glass or plastic tray and support (a
gel casting tray turned upside down), cling film.
.
أعلان
الملكية الفكرية محفوظة لمؤلف الكتاب المذكور فى حالة وجود مشكلة بالكتاب الرجاء الإبلاغ من خلال الايميل الخاص بنا فى صفحة حقوق النشر أو من خلال صفحتنا على الفيس بوك.
وصف كتاب Principles and Protocols Humana Press
2001م - 1443هـ نبذه عن الكتاب:
The isolation of specific regions within the genome of an organism is now
normally accomplished by polymerase chain reaction (PCR) amplification
using primers specific for the region in question. However, there are occasions
where this is not possible (loss of primer sites resulting in no amplification or if
there are no primers available). In these cases the detection of the sequence of
interest can be achieved by hybridization of a labeled probe to restricted
genomic DNA immobilized on a membrane by Southern blotting (1). Genomic
DNA is first digested by one or more restriction enzymes and the fragments
generated separated by gel electrophoresis. The amount of DNA to be applied
to the gel varies from application to application. In general 10 µg of human
genomic DNA is needed for the detection of a single copy gene when using
radioactively labeled probes and an overnight exposure to X-ray film. This
figure can be reduced if the target is either a repetitive element (e.g., ribosomal
DNA) or, if plasmid DNA or PCR products are run on the gel. Once the fragments are separated on the gel the DNA is then denatured in situ and transferred by capillary transfer to either a nitrocellulose or nylon membrane. The
DNA fragments are then bound to the membrane, which can then be used in a
hybridization reaction.
1.2. Materials
1.2.1. Specific Materials
1. 3MM filter paper (Whatman), paper towels, glass or plastic tray and support (a
gel casting tray turned upside down), cling film.
.
أعلان
أعلان
تحميل
| التحميل | حجم الكتاب |
|---|---|
| غير محدد فى الوقت الحالى |
أعلان
أضافة مراجعة
1 (0)
2 (0)
3 (0)
4 (0)
5 (0)
كتب ذات صلة بكتاب Principles and Protocols Humana Press
0.0
كتاب Mineral and Thermal Waters of Southea ..
Petar Papić
كتب منوعة - Biology Books - غير محدد عدد الصفحات
0.0
كتاب Plant life and plant uses an elementa ..
غير معروف
كتب منوعة - Biology Books - غير محدد عدد الصفحات
0.0
كتاب Springer International Publishing
Colby Sawyer College Harvey
كتب منوعة - Biology Books - غير محدد عدد الصفحات
0.0
كتاب Parasitology or Mycology Lecture Guid ..
غير معروف
كتب منوعة - Biology Books - غير محدد عدد الصفحات