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تحميل كتاب Methods in Molecular Biology Expression Protocols pdf
المؤلف : Peter E Vaillancourt
عن الكتاب : 2003م - 1443هـ
نبذه عن الكتاب:
The aim of E. coli Gene Expression Protocols is to familiarize and
instruct the reader with currently popular and newly emerging methodologies
that exploit the advantages of using E. coli as a host organism for expressing
recombinant proteins. The chapters generally fall within two categories:
(1) the use of E. coli vectors and strains for production of pure, functional
protein, and (2) the use of E. coli as host for the functional screening of large
collections of proteins or peptides. These methods and protocols should be of
use to researchers over a wide range of disciplines. Chapters that fall within
the latter category describe protocols that will be particularly relevant for
functional genomics studies.
The chapters of E. coli Gene Expression Protocols are written by experts
who have hands-on experience with the particular method. Each article is
written in sufficient detail so that researchers familiar with basic molecular
techniques and experienced with handling E. coli and its bacteriophages should
be able to carry out the procedures successfully. As in all volumes of the
Methods in Molecular Biology series, each chapter includes an extensive Notes
section, in which practical details peculiar to the particular method are
described.
E. coli Gene Expression Protocols is not intended to be all inclusive, but
is focused on new tools and techniques—or new twists on old techniques—
that will likely be widely used in the coming decade. There are several wellestablished E. coli expression systems (e.g., the original T7 RNA polymerase
expression strains and vectors developed by William F. Studier and colleagues;
the use of GST and polyhistidine fusion tags for protein purification) that
have been extensively described in other methods volumes and peer-reviewed
journal articles and are thus not included in this volume, with the exception of
a few contributions in which certain of these systems have been adapted for
novel applications or otherwise improved upon. .
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وصف كتاب Methods in Molecular Biology Expression Protocols
2003م - 1443هـ
نبذه عن الكتاب:
The aim of E. coli Gene Expression Protocols is to familiarize and
instruct the reader with currently popular and newly emerging methodologies
that exploit the advantages of using E. coli as a host organism for expressing
recombinant proteins. The chapters generally fall within two categories:
(1) the use of E. coli vectors and strains for production of pure, functional
protein, and (2) the use of E. coli as host for the functional screening of large
collections of proteins or peptides. These methods and protocols should be of
use to researchers over a wide range of disciplines. Chapters that fall within
the latter category describe protocols that will be particularly relevant for
functional genomics studies.
The chapters of E. coli Gene Expression Protocols are written by experts
who have hands-on experience with the particular method. Each article is
written in sufficient detail so that researchers familiar with basic molecular
techniques and experienced with handling E. coli and its bacteriophages should
be able to carry out the procedures successfully. As in all volumes of the
Methods in Molecular Biology series, each chapter includes an extensive Notes
section, in which practical details peculiar to the particular method are
described.
E. coli Gene Expression Protocols is not intended to be all inclusive, but
is focused on new tools and techniques—or new twists on old techniques—
that will likely be widely used in the coming decade. There are several wellestablished E. coli expression systems (e.g., the original T7 RNA polymerase
expression strains and vectors developed by William F. Studier and colleagues;
the use of GST and polyhistidine fusion tags for protein purification) that
have been extensively described in other methods volumes and peer-reviewed
journal articles and are thus not included in this volume, with the exception of
a few contributions in which certain of these systems have been adapted for
novel applications or otherwise improved upon. .
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