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تحميل كتاب Methods in Molecular Biology Elias pdf
المؤلف : Elias A Lianos
عن الكتاب : 1999م - 1443هـ نبذه عن الكتاب:
Introduction
Expression of foreign proteins in both cultured and intact mammalian cells
has become essential to evaluating cellular biology and physiology including
the proteins involved in the control of eicosanoid production. Many transient
expression vectors are commercially available and are in widespread use. However, some cell types, such as the renal mesangial cell, have been difficult to
transform with these vectors and require a different strategy to obtain stable
expression of the exogenous gene. Viral vectors, including retroviruses,
adenoviruses, and the Sinbis virus have been developed over the last decade
for foreign gene expression in mammalian cells. This chapter will discuss the
use of a replication-incompetent retroviral vector in cell culture to express an
exogenous protein.
.
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وصف كتاب Methods in Molecular Biology Elias
1999م - 1443هـ نبذه عن الكتاب:
Introduction
Expression of foreign proteins in both cultured and intact mammalian cells
has become essential to evaluating cellular biology and physiology including
the proteins involved in the control of eicosanoid production. Many transient
expression vectors are commercially available and are in widespread use. However, some cell types, such as the renal mesangial cell, have been difficult to
transform with these vectors and require a different strategy to obtain stable
expression of the exogenous gene. Viral vectors, including retroviruses,
adenoviruses, and the Sinbis virus have been developed over the last decade
for foreign gene expression in mammalian cells. This chapter will discuss the
use of a replication-incompetent retroviral vector in cell culture to express an
exogenous protein.
.
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