Name: Chiral Separations Methods and Protocols Humana Press
Author: Gerald Gübitz Martin G Schmid

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تحميل كتاب Chiral Separations Methods and Protocols Humana Press PDF

المؤلف : Gerald Gübitz Martin G Schmid

التصنيف : كتب منوعة

الفئة : Biology Books

سنة النشر : 2003

عدد الصفحات : غير محدد

عن الكتاب : 2003م - 1443هـ نبذه عن الكتاب: Gerald Gübitz and Martin G. Schmid 1. Introduction The development of methods for chiral separation on an analytical as well as on a preparative scale has attracted great attention during the past two decades. Chromatographic methods such as gas chromatography (GC) (1), high-performance liquid chromatography (HPLC) (2–6), supercritical fluid chromatography (SFC) (7–9), and thin-layer chromatography (TLC) (10–13) have been developed using different chiral separation principles. More recently, capillary electrophoresis (CE) (14–21) and capillary electrochromatography (CEC) (22–25) have been shown to be powerful alternatives to chromatographic methods. Several separation principles successfully used in HPLC have been transferred to CE and CEC. For the separation of enantiomers on a preparative scale, LC has become increasingly attractive. The main domain of chromatographic and electromigration techniques is obviously the separation on an analytical scale for enantiomer purity control in synthesis, check for racemization processes, pharmaceutical quality control, pharmacokinetic studies, etc. Chromatographic enantiomer separations can be carried out either indirectly by using chiral derivatization reagents to form diastereomeric derivatives or directly using chiral selectors, which can be incorporated either in the stationary phase or the mobile phase. Similarly, in CE, indirect and direct ways are possible, thereby, in the latter approach, the chiral selector is simply added to the electrolyte. CEC represent a new hybrid method between HPLC and CE. Accordingly, the chiral selector can be present in the mobile phase or in the stationary phase. Open tubular capillaries containing the stationary phase coated to the wall and packed capillaries are used. A new trend is to move away from packed capillaries .

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نبذة عن كتاب Chiral Separations Methods and Protocols Humana Press

كتاب Chiral Separations Methods and Protocols Humana Press

2003م - 1443هـ نبذه عن الكتاب: Gerald Gübitz and Martin G. Schmid 1. Introduction The development of methods for chiral separation on an analytical as well as on a preparative scale has attracted great attention during the past two decades. Chromatographic methods such as gas chromatography (GC) (1), high-performance liquid chromatography (HPLC) (2–6), supercritical fluid chromatography (SFC) (7–9), and thin-layer chromatography (TLC) (10–13) have been developed using different chiral separation principles. More recently, capillary electrophoresis (CE) (14–21) and capillary electrochromatography (CEC) (22–25) have been shown to be powerful alternatives to chromatographic methods. Several separation principles successfully used in HPLC have been transferred to CE and CEC. For the separation of enantiomers on a preparative scale, LC has become increasingly attractive. The main domain of chromatographic and electromigration techniques is obviously the separation on an analytical scale for enantiomer purity control in synthesis, check for racemization processes, pharmaceutical quality control, pharmacokinetic studies, etc. Chromatographic enantiomer separations can be carried out either indirectly by using chiral derivatization reagents to form diastereomeric derivatives or directly using chiral selectors, which can be incorporated either in the stationary phase or the mobile phase. Similarly, in CE, indirect and direct ways are possible, thereby, in the latter approach, the chiral selector is simply added to the electrolyte. CEC represent a new hybrid method between HPLC and CE. Accordingly, the chiral selector can be present in the mobile phase or in the stationary phase. Open tubular capillaries containing the stationary phase coated to the wall and packed capillaries are used. A new trend is to move away from packed capillaries .

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